Fig. 5.
GM-CSF–induced colony formation by primary bone marrow cells after retroviral transduction of YY1.
(A) RNA spot blot analysis of supernatants containing BABE/HA-YY1 or BABE vector control virus, showing that titers used for infection were comparable. The filter was hybridized with a BABE-specific cDNA probe. (B) GM-CFU assay of primary bone marrow progenitor cells following infection with BABE-HA-YY1 or BABE control virus. Bone marrow cells were plated in triplicate at densities of 10 to 50 × 103 cells per dish in 1 mL methylcellulose medium containing GM-CSF (20 U/mL) and puromycin (2.5 μg/mL). Two independent experiments are shown.
