Fig. 4.
MHC class II and CD14 levels on adherent cells from GM-P cultures.
(A) Flow cytometry of MHC class II and CD14 on mock-infected cells at day 15 after infection. (B) Flow cytometry of MHC class II and CD14 on CMV-infected cells at day 15 after infection. (C) Flow cytometry MFI analysis of MHC class II levels on mock-infected and CMV-infected cells at days 2, 5, 8, 12, and 15 after infection on gated CD14+cells. (D) Flow cytometry MFI analysis of CD14 protein levels on mock-infected and CMV-infected cells at days 2, 5, 8, 12, and 15 after infection on gated CD14+ cells. GM-P cultures were infected with CMV strain TownevarRIT3 (MOI of 3) and adherent cells were stained with anti–MHC class II (HLA-DR) antibody TU36 and anti–CD14 antibody TUK4. Grey bars, mock-infected samples; white bars, CMV-infected samples.