Expression of MHC class II protein in adherent cells from GM-P cultures by immunoblot and immunofluorescence analyses.

(Top panels) Immunoblot analysis. (A) MHC class II (HLA-DRα) immunoblot performed on whole cell lysates from mock-infected cells and CMV-infected cells at day 12 after infection. (B) MHC class II (HLA-DRα) immunoblot performed on whole cell lysates from mock-infected cells and CMV-infected cells at day 15 after infection. (Bottom panels) Immunofluorescence analysis of MHC class II protein expression in CMV latently infected cells on day 15 after infection. (C) Mock-infected cells stained with MHC class II antibody IQU9. (D) CMV strain Toledo–infected cells stained with MHC class II antibody IQU9. (E) CMV mutant RV798-infected cells stained with MHC class II antibody IQU9. (F) CMV strain Toledo–infected cells stained with isotype control antibody. (G, H) CMV strain Toledo–infected cell dual stained for MHC class II (G) and CD14 (H). Anti–MHC class II (red staining) and CD14 (green staining) antibodies were detected with TR- and FITC-conjugated secondary antibodies, respectively. Arrow points to the cell surface of a mock-infected cells and arrowheads point to accumulation of MHC class II in latently infected cells. Original magnification, × 600.