Analysis of MHC class II (DR, DP, DQ) and HLA-DM protein localization in CMV latently infected cells.

Immunofluorescence staining of mock-infected (A-C) and CMV-infected cells (D-F) at day 15 after infection. Cells were dual stained for MHC class II (A and D; red staining) and HLA-DM (B and E; green staining). Fluorescent images were digitally overlaid to assess colocalization (C, F). Arrow indicates cell surface periphery MHC class II staining on a mock-infected cell. Original magnification, × 600.