Representative experiment of HA-HABP binding negatively regulating HSC proliferation and differentiation.

(A) Number of progenitors produced per 320 CD34⁺CD38⁻ CB HSCs seeded in serum-free culture for 6 days with various concentrations of HABP. (B) CD15 expression of cells cultured in various concentrations of HABP in Figure 6A. (C-F) CD34 and CD38 expression of cells cultured in various HABP concentrations in Figure 6A. The percentage in each quadrant represents the proportion of cultured cells expressing the particular phenotype. The number in parentheses represents the total number of CD34⁺CD38⁻ cells in the culture compared with the original 320 cells seeded.