Binding of a serotonin conjugate to fibrinogen and thrombospondin.
(A) Microtiter plates coated with fibrinogen, fragment D, and fragment E (10 μg/mL) were incubated with graded concentrations of b-BSA-(5-HT)6. Bound biotin was detected with streptavidin-peroxidase. (B) Plates were coated with thrombospondin and analyzed as in the previous panel. (C) B-BSA-(5-HT)6adsorbed to the plate was incubated with graded levels of fibrinogen and fragment D. Bound ligand was detected with goat antifibrinogen antibody and peroxidase-conjugated rabbit antigoat immunoglobulin G (IgG). (D) Thrombospondin was bound to the plate and then treated with chymotrypsin or buffer as described in “Materials and methods.” B-BSA-(5-HT)6 binding was then determined as detailed for panel A.
