Cloning of ZBP-89.

(A) Nucleotide sequence of the proximal human CD11bpromoter. The major transcription initiation site (arrow) is numbered +1. The GC-rich region is in bold and underlined. (B) Nucleotide sequence of GC (wild-type) and GCm (mutant) oligonucleotides (sense strand). Mutations are indicated in bold underlined letters. (C) Schematic of the primary structure of human ZBP-89 and p25 (see text for details). The vertical numbers indicate the amino acid numbers. The acidic domain is shown as a shaded bar; 2 basic domains as solid bars; 4 zinc finger domains as hatched bars, and a PEST sequence as a dotted bar. A single nucleotide insertion in p25 results in an in-frame premature termination codon after amino acid 284 of ZBP-89.