Antigen-presentation capacity of cultured immature MoDCs.

(A) ³H-Thymidine incorporation (cpm × 10³) in allogeneic MLR induced by MoDCs cultured with (▪) or without (■) the addition of 20 μg/mL recombinant β₂M. (B-C) Cytokines IFN-γ and TNF-α (B), and IL-2 (C) secreted by allospecific T cells activated by MoDCs cultured with (▪) or without (■) the addition of 20 μg/mL of recombinant β₂M. Values above the bars represent the concentration of the indicated cytokines. Cytometric bead array analysis was used to measure cytokine concentrations in the medium of T-cell cultures (containing T cells [Tc] and DCs at a ratio of 100:1) on day 6. (D)³H-Thymidine incorporation (cpm × 10³) in autologous T-cell response induced by PPD-pulsed (squares) or unpulsed (triangles) immature MoDCs cultured with (▪,▴) or without (■,▵) the addition of 20 μg/mL recombinant β₂M. Mean ± SEM of 3 independent experiments are shown. *P < .05; **P < .01.