Phenotypic features of mature MoDCs harvested on day 9.

(A) Cells were cultured for the first 7 days in medium with (shaded) or without (open histograms, thick lines) the addition of 20 μg/mL recombinant β₂M, followed by an additional 48-hour culture in the presence of IL-1β and TNF-α. Open histograms with thin or broken lines represent control antibody staining of the cells. (B) Dot plots of flow cytometry analysis showing the effects of β₂M during maturation (days 7-9) of previously (days 0-7) β₂M-treated or untreated DCs. Representative histograms or dot plots of 5 independent experiments are shown. Gates were set on the large-size cell populations.