Expression of MAPKs by MoDCs.

Culture in medium with (+β₂M) or without (−β₂M) the addition of 20 μg/mL recombinant β₂M. (A) Western blots were made with cell lysates collected on day 1 (D1), day 3 (D3), and day 7 (D7) of the culture. MAPKs ERK1/2 and MEK1/2 were immunoblotted with specific antibodies. Phosphorylated (pERK1/2 and pMEK1/2) and nonphosphorylated (ERK1/2 and MEK1/2) MAPKs were detected. The latter could also serve as control for protein loading. Representative blots are shown. (B) Densitometric data (AVG) of pERK1/2 and pMEK1/2 represent the mean ± SEM of 3 independent experiments.