Expression of IκB and NF-κB and STAT3 by MoDCs cultured in medium with (+β₂M) or without (−β₂M) the addition of 20 μg/mL recombinant β₂M.

(A) Western blots were made with cell lysates collected on day 1 (D1), day 3 (D3), and day 7 (D7) of culture. IκB-α and IκB-β, phosphorylated IκB-α (pIκB-α), STAT3, and phosphorylated STAT3 (pSTAT3) were immunoblotted with specific antibodies. NF-κB activity was analyzed by EMSA. Representative blots are shown. (B) Densitometric data (AVG) of pIκB-α and IκB-α represent the mean ± SEM of 3 independent experiments.