Fig. 2.
PMA-induced calcium influx is dependent on extracellular calcium.
(A) Cell suspensions of Fluo-3/am–loaded RBCs were treated with 3 μM PMA, 1 μM A23187, or DMSO in the absence of Ca2+ and examined on an individual cell basis by flow cytometry. (B) Alternatively, the cells were restored to normal extracellular Ca2+ levels (2 mM) 5 minutes after stimulation with PMA, A23187, or DMSO, as described above. Representative tracing from one donor is shown; tracing was performed in duplicate.
