1,2-didecanoyl-rac-glycerol (DAG) stimulates calcium influx into Fluo-3/am–loaded RBCs similar to PMA.

Cell suspensions of Fluo-3/am–loaded RBCs were stimulated with (A) 3 μM PMA or (B) 10 μM or (C) 5 μM 1,2-didecanoyl-rac-glycerol dissolved in DMSO, and fluorescence was monitored by fluorescence spectroscopy. Negative controls included (D) 4α-phorbol and (E) DMSO. Tracings representative of 3 different donors are shown. Arrow designates time of addition of stimulus.