PMA- and DAG-treated RBCs decrease in cell size after treatment.

(A) RBCs treated with 2 μM A23187 (thin line), 3 μM PMA (thick line), or DMSO (control; dotted line) were incubated for 40 minutes at 37°C and compared for changes in cell volume by forward light scattering using a flow cytometer. (B) The same experiment as in (A) was performed, except that the DAG analog 1,2-didecanoyl-rac-glycerol (thick line) was incubated at 5 μM. Tracings representative of 3 different donors are shown.