PMA-treated RBCs exhibit resistance to hypotonic lysis compared with DMSO (control)-treated RBCs.

RBCs (HCT 10%) were incubated with either 3 μM PMA or DMSO as control for 40 minutes at 37°C in a shaking water bath. Osmotic fragility was then assessed using solutions of 0, 30, 60, 75, 90, 105, 120, 135, 150, and 300 mOsm. The hemoglobin concentration in the supernatant from each test solution was determined using Drabkin reagent (Sigma, St Louis, MO). The data points represent the mean ± SD from 2 different donors with 3 replicates per person.