Flow cytometric analysis of cell surface phenotype of cells derived from human muscle and neural tissues.
Flow cytometry was used to evaluate the cell surface expression of the human-specific proteins associated with hematopoietic tissue: AC133, and cell differentiation markers CD34 and CD45. A representative FACS analysis of de novo–isolated muscle (A) and neural (B) cells is shown, and the percentage of subpopulations expressing single or coexpressing human hematopoietic markers is shown as the average mean ± SEM (n = 4) in each quadrant. Specificity of hematopoietic antibody staining and background signal was determined by comparing muscle and neural cells stained with mouse IgG1 (Ai and Bi) to establish positive quadrant levels to omit fluorescence because of nonspecific binding and auto fluorescence properties of the cells. Results are based on data from 4 independent samples of muscle and neural tissues analyzed in duplicate.
