Evaluation of human hematopoietic cell fate potential of de novo and cultured muscle and neural cells.

Primary human muscle (A) and neural (B) tissues were examined for hematopoietic progenitor capacity (CFU) at isolation (day 0) or after 5 days of in vitro culture in essential media containing 10% CEE, or human hematopoietic growth factors (HGF) alone or with BMP-4 or EPO, as single additions or in combination as indicated. In all culture conditions, media and cytokines were replenished every other day. Single, double, and triple asterisks indicate substantial differences within measured groups of P < .01. Data shown is based on 6 to 13 independent samples analyzed in culture conditions indicated.