Phosphorylation of MAPK/ERK1,2 is up-regulated in the presence of BMSCs.

INA-6 cells were cultivated without IL-6 (lane 1), with 2 ng/mL IL-6 (lanes 2 and 3), or with BMSCs (lanes 4 and 5) and treated with Sant7 (50 μg/mL; lanes 3 and 5) for 12 hours. Western blot analysis with a Thr202/Tyr204-phosphospecific anti-ERK1,2 antibody was performed to determine the phosphorylation status of ERK1,2 (pERK1,2) in INA-6 cells. Western blot analysis of the unphosphorylated ERK1,2 served as a loading control.