Development and expression specificity of β-globin transgenic mice.

(A) Diagram of construct used to make the transgenic line. HS indicates DNAse-1 hypersensitive sites within the β-globin LCR; pβ, β-globin promoter. (B) Peripheral blood from a β-globin transgenic (Tg) mouse (line no. 3) and a wild-type (WT) control, viewed under brightfield or fluorescence microscopy. Original magnification, × 20. The second panel showing fluorescent transgenic erythrocytes represents a higher magnification (× 5 of the original). (C) FACS analysis of the peripheral blood from β-globin ECFP transgenic mice (line nos. 1, 2, 3) and a wild-type (WT) control. (D) FACS profiles of hematopoietic progenitors showing ECFP expression. HSCs were sorted as IL-7Rα⁻Lin⁻Sca-1⁺c-Kit⁺; CMPs as IL-7Rα⁻Lin⁻Sca-1⁻c-Kit⁺, CD34⁺, FCgR^l0; GMPs as IL-7Rα⁻Lin⁻Sca-1⁻c-Kit⁺, CD34⁺FCgR^hi ; MEPs as IL-7Rα⁻Lin⁻Sca-1⁻c-Kit⁺, CD34⁻FCgR^l0; CLPs as IL-7Rα⁺Lin⁻Sca-1^loc-Kit⁺; pro–T cells as CD4^loCD8^loCD25⁺c-Kit⁺, and pro–B cells as B220⁺IgM⁻CD43⁺ populations. Numbers indicate percentages of ECFP-positive cells detected within a given quadrant.