Methylcellulose colonies from β-globin ECFP mouse bone marrow.

(A,C) Overlay between fluorescence and brightfield images. (B,D) Brightfield images of the same colonies after benzidine staining, with positive cells shown in black. The colony shown in panels A and B is erythroid, and the one in C and D is myeloid. Some cells or clusters within the colonies have shifted position after staining with benzidine. Original magnification, × 10.