Analysis of hematopoietic cells from a β-globin ECFP x lysozyme EGFP mouse.

(A-B) Overlay of fluorescence microscopic images obtained with ECFP and EYFP filters from blood and bone marrow, respectively (in B, the brightfield image also was included). (C-D) FACS profiles of the same cells. Cells within the gates highlighted in the SSC/FSC plots were analyzed with a laser/filter configuration that allows to distinguish between ECFP and EGFP fluorescence. SSC indicates side scatter; FSC, forward scatter; ECFP and EGFP, log fluorescence intensity in the respective channels. Numbers indicate percentages of cells within a given quadrant. (E) Mixed colony in methylcellulose culture, photographed as in panel B. In addition to numerous small cyan fluorescent (erythroid) and large green fluorescent (myeloid) cells, the colony contains a very large unlabeled cell, possibly a megakaryocyte. Original magnifications: × 20 (A-B, E).