Fig. 1.
Genomic sequence of the hMIP-1α promoter.
The DNA sequence of a 2-kb hMIP-1α promoter was determined using the NCBI database. EcoRI (−1995: gaattc) andXhoI (+54: ctcgag) sites (in bold) were used for subcloning into luciferase reporter constructs. Transcriptional starting site (+1) and translational starting Met site (+98:atg) were determined by Nakao et al,14and the MscI site (−1275: tggcca) was used for Erase-A-Base studies.