Generation of human MIP-1α reporter constructs.

Truncated forms of hMIP-1α promoter were generated from the 2-kb pGL2E-MIP construct by the Erase-A-Base system and followed by sequence analysis to confirm the 5′ end of the hMIP-1α promoter. These luciferase reporter constructs were transfected into the various cell lines to assess MIP-1α promoter activity.