Transduction of AML-1B cDNA into ARH-77 and MM.1S cells totally blocked MIP-1α expression.

AML-1A, AML-1B cDNA, or pcDNA3 vector were stably transfected into the ARH-77 (A) and MM.1S (B) cells and expression levels of MIP-1α were measured using an ELISA kit. Transduction of AML-1B cDNA markedly decreased MIP-1α production in ARH-77 and MM.1S cells (< 200 pg/mL) and transduction of AML-1A cDNA did not significantly alter MIP-1α expression. Similar results were seen in 2 independent experiments (*P < .05).