Fig. 5.
B-lineage characteristics are established by the CD10+ stage in adult BM.
Expression of lineage-related genes was examined in the CD34+Lin−CD10−CD7−(▪), CD34+Lin−CD10−CD7+ (▨), and CD34+Lin−CD10+CD7−(■) fractions sorted from adult BM. RT-PCR was used to amplify transcripts for the indicated genes, and the results were normalized according to GAPDH expression.