Increased PI3-kinase subunit p110 protein levels in neutrophils from MDS patients.

(A) Neutrophils from healthy donors (n = 5) and MDS patients (n = 7) were resuspended in 1 × Laemmli, and samples were boiled. Proteins were separated by SDS-PAGE, and Western blotting was performed using an antibody against PI3-kinase p110 (upper panel). Equal loading was demonstrated by reprobing the blots with an antibody against ERK1/2 (lower panel). (B) Protein levels were quantified by densitometry of the films. The p110 protein levels were corrected for ERK levels, and differences between the means of the normalized values were calculated between MDS neutrophils and healthy donors using the Studentt test for unpaired samples. Significant differences are indicated with an asterisk (P < .05).