GM-CSF stimulates STAT5 activation in neutrophils from MDS patients and healthy controls to a similar extent.

Cell lysates from unstimulated neutrophils (−) and neutrophils that were stimulated for 10 minutes with 5 ng/mL GM-CSF (+) were analyzed for 3 MDS patients and 3 healthy volunteers. Western blot analysis was performed, and proteins were detected using an antibody against phosphorylated STAT5 (upper panel). Equal loading was confirmed by reprobing the blot with antibodies against ERK1/2.