HMVECs transfected with the Akt dominant-negative mutant or Src antisense oligonucleotides showed significantly less sE-selectin–mediated chemotaxis.

(A) HMVECs were transiently transfected with pcDNA3 plasmids containing ERK1/2 or Akt dominant-negative mutants. Plasmids with no insert were used as a negative control. At 72 hours after transfections, HMVEC chemotaxis was performed. (B) HMVECs were transiently transfected with c-Src antisense ODN or control sense ODN. At 16 hours after transfections, HMVEC chemotaxis was performed. Results are expressed as the mean number of cells migrating through the membrane per well ± SEM from 4 independent experiments. *Represents a significant difference (P < .05) between the respective groups. HMVECs transfected with an Akt mutant or Src antisense ODN showed significant inhibition of sE-selectin–mediated chemotaxis, whereas HMVECs transfected with an ERK1/2 mutant failed to show significant inhibition.