sE-selectin induces endothelial cell tube formation on Matrigel in vitro.

The Matrigel in vitro HMVEC tube formation assay was performed in 8-well chamber slides. (A) Photomicrographs of representative assays for DMSO; bFGF; sE-selectin; sE-selectin + PP2; sE-selectin + LY294002; and sE-selectin + PD98059. Original magnification, × 20. (B) Quantitative data for endothelial cell tube formation are expressed as number of tubes/well ± SEM from 3 independent experiments. bFGF is used as positive control. *Represents a significant difference (P < .05) between the respective groups. sE-selectin induced significantly more endothelial tube formation than vehicle control, and this tube formation was inhibited upon treatment with PP2 and LY294002. However, HMVECs treated with PD98059 did not show significant inhibition of sE-selectin–induced endothelial cell tube formation.