Separation of the lipid PMN priming activity from the plasma of TRALI patients before transfusion, at the time of blood typing, and after transfusion, at the time TRALI was recognized, as a function of the retention time of lipids separated by normal phase HPLC.

Lipids were extracted from paired pretransfusion (●) and posttransfusion (▪) plasma samples, separated by normal-phase HPLC, resolubilized in 1.25% albumin, and tested for their ability to prime the fMLP activation of the oxidase of PMNs from healthy donors compared with albumin-treated controls. Two peaks of PMN priming activity are present at the retention times of neutral lipids and lyso-PCs that are not present before transfusion. * indicates statistical significance (P < .05) between the pretransfusion plasma and posttransfusion plasma groups. The sample size is 6 for both groups.