Hematopoietic activity of IL-5trg bone marrow in vitro.
LTBMCs (n = 3 per group) were established from IL-5trg and WT mice. (A) Photographs of adherent layers from IL-5trg and WT LTBMCs were taken after 4 weeks of culture. Areas of hematopoiesis are evident in WT but not in IL-5trg LTBMCs. Magnification ×10. (B) Half of the culture medium was harvested from LTBMCs after each feeding, and the number of nonadherent cells was calculated and expressed as mean ± SD. (C) Nonadherent cells harvested from IL-5trg and WT LTBMCs were plated at a concentration of 1 × 104 cells/mL in the presence of GM-CSF (10 ng/mL). After 7 days of culture, the number of colonies was counted, recalculated for the total number of nonadherent cells recovered from LTBMCs, and expressed as mean ± SD of the number of CFCs.
