American Society of Hematology

Fig. 4.

$Fig. 4. IVIg renders DCs refractory to LPS-mediated maturation. / (A) DCs were generated as described in “Materials and methods.” At day 5, immature DCs were treated with IVIg (0.15 mM) for 12 hours followed by stimulation with LPS (1 μg/mL) (middle row) or were treated with LPS alone (top row) for 48 hours. In control experiments, cells were treated with HSA as an irrelevant protein for 12 hours followed by stimulation with LPS (1 μg/mL; bottom row). Percentages of cells positive for the indicated markers are depicted in upper right corners. Dark histograms represent isotype control. (B) In a second set of experiments, immature DCs were stimulated with LPS (1 μg/mL) for 3 hours followed by incubation with IVIg (bottom row) or without IVIg (top row) for 48 hours. Percentages of cells positive for the indicated markers are depicted in upper right corners, and MFI is indicated in parentheses. Results shown are representative of 4 independent experiments from different donors. (C) Effect of different sources of IVIg on LPS-mediated maturation of dendritic cells. Monocyte-derived DCs were stimulated with LPS (1 μg/mL) for 3 hours followed by incubation with Gammagard, Endobuline, Intraglobin, and Sandoglobulin (0.15 mM) for 48 hours. The percentage down-regulation of MFI of surface molecules on DCs following IVIg treatment is shown. LPS-treated DCs represented 100% expression. Data are represented as means and standard deviations calculated from 2 independent experiments from different donors.$

IVIg renders DCs refractory to LPS-mediated maturation.

(A) DCs were generated as described in “Materials and methods.” At day 5, immature DCs were treated with IVIg (0.15 mM) for 12 hours followed by stimulation with LPS (1 μg/mL) (middle row) or were treated with LPS alone (top row) for 48 hours. In control experiments, cells were treated with HSA as an irrelevant protein for 12 hours followed by stimulation with LPS (1 μg/mL; bottom row). Percentages of cells positive for the indicated markers are depicted in upper right corners. Dark histograms represent isotype control. (B) In a second set of experiments, immature DCs were stimulated with LPS (1 μg/mL) for 3 hours followed by incubation with IVIg (bottom row) or without IVIg (top row) for 48 hours. Percentages of cells positive for the indicated markers are depicted in upper right corners, and MFI is indicated in parentheses. Results shown are representative of 4 independent experiments from different donors. (C) Effect of different sources of IVIg on LPS-mediated maturation of dendritic cells. Monocyte-derived DCs were stimulated with LPS (1 μg/mL) for 3 hours followed by incubation with Gammagard, Endobuline, Intraglobin, and Sandoglobulin (0.15 mM) for 48 hours. The percentage down-regulation of MFI of surface molecules on DCs following IVIg treatment is shown. LPS-treated DCs represented 100% expression. Data are represented as means and standard deviations calculated from 2 independent experiments from different donors.

This Feature Is Available To Subscribers Only