Representative confirmation analysis by real-time quantitative RT-PCR and Western blot.
(A) Representative plots confirming alterations in expression of select genes in plasma cells (▪) versus IgM+ B cells (⋄) by real-time quantitative RT-PCR are shown. ΔRn represents the reporter signal with the baseline subtracted. The threshold cycle (CT) values for these genes as well as additional genes are listed in Table 1. The threshold is indicated by the dotted line. (B) The expression of TBP protein in plasma cells and IgM+ B cells was determined by Western blot. The J558 murine plasmacytoma cell line was used as a positive control for TBP. To control for sufficient protein in plasma cell samples, lysates were examined for the presence of IgG using a polyclonal goat antimouse IgG antibody.
