(A) Rate of pseudopod extension for cells incubated in the presence of increasing concentrations of wortmannin.
The rate of extension is independent of wortmannin concentration above 500 nM. (B) Transmitted light and actin-stained fluorescent images of: (i-ii) passive neutrophil; (iii-iv) neutrophil with a pseudopod stimulated with 10−7 M fMLP; (v-vi) neutrophil with fMLP-stimulated pseudopod in buffer containing 1 μM wortmannin; and (vii-viii) neutrophil with fMLP-stimulated pseudopod in buffer with 20 μM PP2. Bar = 5 μm. (C) Average rates of pseudopod extension for cells incubated with different inhibitors and combinations of inhibitors. Each rate is the average from at least 10 cells. The inhibitors used were 1 μg/mL pertussis toxin; 1 μM wortmannin (WTM); 10 μM chelerythrine chloride; 40 μM Akt-inhibitor; 10 μM diphenyleneiodonium chloride (DPI); 20 μM PP2; 200 μM dibutyryl cyclic-AMP (dBcAMP); 20 μg/mL Clostridium botulinum C3 exoenzyme (C3); 10 μM Y-27632. The statistical significance for all measurements is P < .01, compared with control, calculated using the one-way analysis of variance test.
