Regulation of proteins involved in the control of apoptosis.

(A) Nalm-6 cells were cultured with Fn or PLL for 1, 2 or 3 days before lysis, in absence or presence of serum. An aliquot of each cell lysate was then analyzed by Western blot using anti-Requiem and anti-GAPDH antibodies. (B) Nalm-6 cells were cultured with Fn or PLL for 1, 2, 4 or 5 days in absence of serum before lysis. An aliquot of each cell lysate was then analyzed by Western blot using anti–total and anti–cleaved caspase 7 antibodies as well as anti-GAPDH antibodies. (C) An aliquot of Nalm-6 cell lysate was analyzed by Western blot using anti–caspase 3 and anti-GAPDH antibodies. (D) REH cells were cultured with Fn or PLL for 1, 2, 3 or 5 days before lysis. An aliquot of each cell lysate was then analyzed by Western blot using anti-XIAP, antisurvivin, anti–cleaved caspase 3 and anti-GAPDH antibodies. (E) Nalm-6 cells were cultured on Fn or PLL coated plates for one day with doxorubicin (0.5 μg/mL) before lysis. An aliquot was then analyzed with anti–cleaved caspase 3 or 7, as well as GAPDH antibodies.