The expression of IKK2dn or NIKdn in immature DCs does not affect their viability or differentiation state.

Immature DCs were left uninfected or infected with Adβ-gal, AdNIKdn, AdIKK2dn, or AdIκBα at a MOI of 100. (A) After 2 days, cells were examined for viability by PI staining and FACS. Late apoptotic and dying cells stain positive in this assay. (B) After 2 days, cells were stained for cell surface expression of CD80, CD86, HLA-A, -B, -C, and DR and examined by FACS. DCs stained with isotype control (filled histograms) or specific antibody (open histograms) are compared. The results of 1 experiment representative of 3 independent experiments performed on samples from unrelated donors are shown.