![Fig. 3. The allogeneic MLR is inhibited by overexpression of IκBα or IKK2dn but not NIKdn, but this effect is lost after fixation of DCs. / Immature DCs were left uninfected (white) or were infected with Adβ-gal (gray), AdNIKdn (cross-hatched), AdIKK2dn (checkered), or AdIκBα (black). A MOI of 100 was used. (A) DCs were then plated in graded doses with 105 purified, allogeneic T cells in quadruplicate in a 96-well microtiter plate. (B) DCs were first fixed with 1% paraformaldehyde and then plated in graded doses with 105 purified, allogeneic T cells in quadruplicate in a 96-well microtiter plate. Proliferation was determined on day 6 using the [3H]thymidine uptake assay. Each point represents the mean ± SEM from 5 (A) or 4 (B) independent experiments performed on samples from unrelated donors. Statistical significance was assessed by using a one-tailed Student t test using uninfected cells as a control (*P < .05, **P < .01).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/3/10.1182_blood-2002-06-1835/4/h80333721003.jpeg?Expires=1735524318&Signature=IaVx61JQOz0Wcy83c3qlboy3kKlA--JHk2xMZ6mMFMInkKkL6choD4oHTYOu3z1ovjOiW7lWvk21Jsc4i9bkHnudvA9GfnMhf1oT0F6tfsNzEWM~V4LlciCiDus6XB5aOxYlvrKMjSm~0PCEuPsAPnAfv4Yq5M98QQK8-RKsbsRqKVb9-zJSLYXKWXD8LJQTvRRf9IUgnX02HrI8ivcl9OFqXh00MB0TUDR1ff~z~swM5PxW8zRVJ6Jiqw5mc2CwKVhHYDHOoJ~Rk8gC0aJL7XH8rhrPJd1GKNobxImeAqw0xAb2PhQX-15Du5HmyXOvjOCb6EE8Xvl~PpwMbxviXw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The allogeneic MLR is inhibited by overexpression of IκBα or IKK2dn but not NIKdn, but this effect is lost after fixation of DCs.
Immature DCs were left uninfected (white) or were infected with Adβ-gal (gray), AdNIKdn (cross-hatched), AdIKK2dn (checkered), or AdIκBα (black). A MOI of 100 was used. (A) DCs were then plated in graded doses with 105 purified, allogeneic T cells in quadruplicate in a 96-well microtiter plate. (B) DCs were first fixed with 1% paraformaldehyde and then plated in graded doses with 105 purified, allogeneic T cells in quadruplicate in a 96-well microtiter plate. Proliferation was determined on day 6 using the [3H]thymidine uptake assay. Each point represents the mean ± SEM from 5 (A) or 4 (B) independent experiments performed on samples from unrelated donors. Statistical significance was assessed by using a one-tailed Student t test using uninfected cells as a control (*P < .05, **P < .01).
