Analysis of sorted CD45⁺long-term HSCs.

(A) Results of reanalysis of purified CD45⁺ HSCs. Lin⁻Sca-1⁺c-Kit⁺CD34⁻CD45⁺cells were double sorted and reanalyzed. The purity of the CD45⁺ HSCs reached up to 99% as indicated. (B) Results of RT-PCR assays of nonhematopoiesis-affiliated genes (SBP-1, GnRH, N-RAP, and Phox2) and GATA-2targeting limited numbers of CD45⁺ HSCs. Hypoxanthine guanine phosphoribosyl transferase (HPRT) was used as a positive control.