Fig. 4.
Enhancement of RTX-mediated killing by mAb 3E7.
Raji cells in medium ± RTX, or in 50% NHS ± RTX ± mAb 3E7 at 10 μg/mL, were incubated for varying periods at 37°C in 5% CO2 and stained with FITC annexin V and propidium iodide (PI). Forward and side scattering were used to identify the population of cells; debris generated as a consequence of cell lysis was excluded. Aggregated cells, live cells, and dead cells are indicated by arrows marked A, L, and D, respectively. The percentage of cells in each quadrant (live, lower left; dead, upper right) is displayed. The number of live cells at 1 hour and 48 hours for medium ± RTX as well as for RTX ± mAb 3E7 samples in serum is shown in Figure 5A-B.
