Purification and analysis of AML1-ETO–transduced erythroid progenitors.

Representative density plots (dark shading indicates highest density) show the immunophenotypic status of AML1-ETO–transduced cultures. (A) Following retroviral transduction (day 3). (B) Immunophenotype on day 6 following enrichment of GFP⁺CD13⁻ cells. Data in the right panels exclude myeloid (CD13⁺) cells. Quadrants delimit background fluorescence of control-stained/mock-transduced cells. Similar data were generated from control cultures expressing GFP alone (not shown). FSC indicates forward scatter. Note that the intensity of GFP expression increased in cells expressing AML1-ETO over days 3 to 6.