Elevated iNOS expression in

Fancc^−/− macrophages stimulated with IFNγ and LPS. Peritoneal or bone marrow–derived macrophages were stimulated for 0 to 12 hours with IFNγ (10 ng/mL) with or without LPS (100 ng/mL), and whole cell lysates were assayed for iNOS expression by immunoblotting. (A) Representative filter showing iNOS protein expression in Fancc^−/−and control peritoneal macrophages following IFNγ plus LPS stimulation (top panel), with α-tubulin (bottom panel) as the loading control. (B) Densitometric representation of 5 independent experiments showing a significant difference in iNOS expression betweenFancc^−/− (■) and wild-type (♦) peritoneal macrophages at 8 and 12 hours after stimulation (P = .02, .04, respectively). (C) Representative filter showing iNOS expression in BMDMs following IFNγ stimulation (top panel), with α-tubulin (bottom panel) as the loading control. (D) Densitometric representation of 4 independent experiments showing a significant increase in iNOS expression within Fancc^−/− BMDMs at 5 hours after stimulation, with expression of iNOS reaching a maximum at 8 hours. *P < .05.