Flow cytometric analysis of lineage markers on human hematopoietic cells engrafted in NOD/SCID mice.

For 4 weeks, CD34⁺ cells were expanded on primary stromal cells (A), expanded on hTERT-stromal cells (B), or not expanded (precocultured; C), followed by transplantation into NOD/SCID mice. Hematopoietic cells were immunolabeled with FITC-conjugated hCD45 antibody to ensure human origin and with PE-conjugated antibody specific for the indicated lineage marker. Data shown are from 1 experiment representative of 5 showing similar results.