Fig. 7.
Fas-costimulated and control-activated T cells express equal levels of c-FLIP, Bcl-XL, and Bcl-2 after initial activation.
To evaluate the effect of Fas costimulation on expression of antiapoptotic molecules, RT-PCR was run with specific primers for c-FLIP, Bcl-XL, and Bcl-2 for RNA from freshly isolated (unstimulated) T cells and after 6 days of stimulation. Similar results were obtained in 2 separate PCR reactions with 2 different sets of RNA samples.
