Morphologic characterization of adherent cells.

(A) CD133⁺ cells mobilized from peripheral blood were enriched by MACS. Flow cytometry showed that the purity of the positively selected CD133⁺ cells was generally greater than 99.5%. (B) Inverse microscopic image of typical 6-week-old adherent cells (original magnification, × 200). B indicates bud; M, magnupodium; T, tenupodium. (C) Raster electron micrograph of a 6-week-old adherent cell (original magnification, × 2000). B indicates a bud on the cell surface; L, a spoonlike lobopodium at the terminus of the cell (upper left corner of the micrograph). (D) A 6-week-old adherent cell that emerged from a population of EGFP-expressing CD133⁺ cells is counterstained with PE-conjugated mouse antihuman CD133 antibody. Yellow fluorescence caused by the overlay of green (EGFP) and red (CD133) fluorescence indicates double staining of the buds (indicated by B).