Phenotypic characterization of adherent cells.

Six-week-old adherent cells were treated with trypsin, harvested, washed once with PBS, incubated with mouse monoclonal antibodies to various human antigens, washed twice with PBS, and analyzed by flow cytometry. (A) The cells were negative for CD34, CD133, CD90, CD140b, and KDR. However, they expressed high levels of CD172a, CD164, and the antigen detected by the monoclonal antibody W7C5. (B) Adherent cells were negative for CD50, but expressed considerable levels of VE-cadherin and CD106. In addition, these cells expressed moderate levels of CD49d and high levels of CD44 and CD54 adhesion molecules. Filled histograms show isotype control IgG staining profile, whereas open histograms show specific antibody staining profile.