Analysis of the differentiation potential of EU12 cells.

(A) Subpopulations of EU12 cells were purified by 2 rounds of FACS on the basis of their cell surface expression of CD34 and μHC. The purified EU12 subpopulations were cultured and their cell surface profiles reanalyzed on day 0 (D0), day 1 (D1), day 8 (D8), day 12 (D12), and day 20 (D20). (B) Single EU12 pro-B cells (CD34⁺μHC⁻) were cultured in individual wells and their progeny were characterized by immunofluorescence analysis at week 2 (W2), week 6 (W6), and week 10 (W10).