Expression of B lineage–specific genes in the EU12 subpopulations.

(A) Each subpopulation of EU12 cells was purified by 2 rounds of FACS. (B) RNA obtained from each subpopulation was used in RT-PCR amplification of B lineage–specific genes as described in “Material and methods.” PCR products were visualized by ethidium bromide staining of agarose gels.