American Society of Hematology

Fig. 6.

$Fig. 6. Genomic PCR assay of Ig HC and LC genes in EU12 subpopulations. / The diagram (A) indicates the location of each oligonucleotide used in the PCR reactions. gDNA from unfractionated EU12 cells, each subpopulation of EU12 cells, and control HepG2 cells was used as the template for PCR amplification by primers DXP′1 (internal), and JH6 (B), DXP′1 (external), and JH5 (C), and PanVHFR3, and JH5 (D) for Ig HC gene and a set of primers for Igλ LC genes (E). PCR products were subjected to electrophoresis and blotted with probes JH5 (B), and DXP′1 (internal) for panels C and D or revealed with ethidium bromide staining of agarose gels (E).$

Genomic PCR assay of Ig HC and LC genes in EU12 subpopulations.

The diagram (A) indicates the location of each oligonucleotide used in the PCR reactions. gDNA from unfractionated EU12 cells, each subpopulation of EU12 cells, and control HepG2 cells was used as the template for PCR amplification by primers DXP′1 (internal), and J_H6 (B), DXP′1 (external), and J_H5 (C), and PanV_HFR3, and J_H5 (D) for Ig HC gene and a set of primers for Igλ LC genes (E). PCR products were subjected to electrophoresis and blotted with probes J_H5 (B), and DXP′1 (internal) for panels C and D or revealed with ethidium bromide staining of agarose gels (E).

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