hP2X₁ overexpression in transgenic mouse platelets.

(A) RT-PCR performed on total RNA extracted from platelets or total leukocytes (WBCs) of wild-type (WT) or transgenic (TG) mice. Identical results were found for 2 independent transgenic founder lines. Partial sequences of the human and mouse P2X₁ cDNAs are shown. Primers used to specifically amplify the hP2X₁ and mP2X₁ cDNAs are underlined and in boldface type. The translation initiation codon is boxed. (B) Western blotting detection of the hP2X₁ protein in TG platelet whole-cell lysate with a polyclonal anti-hP2X₁ antibody.12 The hP2X₁ expression levels in identical numbers of platelets from heterozygous or homozygous transgenic mice originating from 2 independent transgenic founder lines (TG₁₆ and TG₁₈) are shown in parallel with those of the endogenous mP2X₁ detected in WT mouse platelets.