Apoptosis rate and cytotoxic activity of B6^dom1-specific T cells in B10 versus B10.H7^b hosts.

BM cells (10⁷) and spleen cells (5 × 10⁷) from B10.H7^b donors preimmunized with B10 splenocytes on day −14 were injected into irradiated (10 Gy) B10 or B10.H7^b recipients on day 0. Recipients were injected with 5 × 10⁴ EL4 cells on day +1. (A) Proportion of Annexin V⁺ cells among tetramer⁺ and tetramer⁻ CD8 T cells from B10 and B10.H7^brecipients. (B) Tetramer staining of CD8 T cells from B10 and B10.H7^b mice; numbers indicate the mean fluorescence intensity of tetramer⁺ cells. (C) Direct cytotoxic activity of CD8 T cells harvested on day 15 after AHCT. Spleen CD8 T cells were enriched by negative depletion of B and CD4 lymphocytes and were cultured for 6 hours at various effector-to-target ratios with [³H]thymidine–labeled EL4 cells (3 mice per group).